《植物生理学报》 2013, 49(5): 457-462

低温下4种秋菊叶片和根系膜脂脂肪酸组分比较

李永华, 史春会, 李永, 杨秋生^*

河南农业大学林学院, 郑州450002

通信作者：杨秋生；E-mail: qsyang@henau.edu.cn；Tel: 0371-63555758

摘　要：

本文应用气相色谱技术, 以早秋菊‘太平的小鼓’、 ‘金锋铃’和晚秋菊‘星光灿烂’、‘墨宝’4个菊花品种为试材, 研究低温处理下叶片和根系中的膜脂脂肪酸组分与含量的变化。结果表明: 4种秋菊叶片和根系中膜脂脂肪酸种类主要有棕榈酸(C16:0)、亚油酸(C18:2)和亚麻酸(C18:3), 叶片中不饱和脂肪酸以亚麻酸(C18:3)为主, 根系中以亚油酸(C18:2)为主, 分别占总脂肪酸含量的49.81%和35.49%以上, 饱和脂肪酸以棕榈酸(C16:0)为主。低温有利于不饱和脂肪酸的形成和IUFA值的提高, 在零下低温时, 晚秋菊IUFA值受温度影响较为显著。叶片中不饱和脂肪酸含量随温度降低逐渐增加, 整个处理过程中叶片不饱和脂肪酸含量远高于根系的, 叶片和根系在膜脂低温响应上存在一定差异。

关键词：菊花; 低温; 不饱和脂肪酸; 叶片; 根系

收稿：2012-12-10   修定：2013-03-28

资助：河南省重大科技专项(091100110200)。

Comparison of Membrane Fatty Acids Components in Leaf and Root of Four Autumn Chrysanthemum Cultivars under Low Temperature

LI Yong-Hua, SHI Chun-Hui, LI Yong, YANG Qiu-Sheng*

College of Forestry, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: YANG Qiu-Sheng; E-mail: qsyang@henau.edu.cn; Tel: 0371-63555758

Abstract:

Early-flowering cultivars ‘Taipingdexiaogu’, ‘Jinfengling’ and late-flowering cultivars ‘Mobao’, ‘Xingguangcanlan’ were used as experimental materials in this paper, components and contents of fatty acid in leaf and root under different temperatures were determined by gas chromatography technology. The results showed that membrane fatty acid in leaves and roots of 4 autumn chrysanthemum cultivars were mainly com-posed of palmitic acid (C_16:0), linoleic acid (C_18:2) and linolenic acid (C_18:3), the main component of unsaturated fatty acid was linolenic acid (C_18:3) in leaves, and linoleic acid (C_18:2) in roots, the contents of which were more than 49.81% and 35.49% in total fatty acids, respectively. The palmitic acid (C_16:0) was main kind of saturated fatty acid. Low temperature contributed to the formation of unsaturated fatty acids and the increament of IUFA, the IUFA of late-flowering cultivars were more significantly affected by subzero low temperature. Unsaturated fatty acids contents in leaves ascended gradually with the decline of temperature, unsaturated fatty acid contents in leaves were higher evidently than those in roots in whole experiments, there were some differences in mem-brane lipids response to low temperature in leaf and root.

Key words: Dendranthema×grandiflorum; low temperature; unsaturated fatty acids; leaf; root